Nanoparticles and Pop-off Technique for Electron Microscopy

Nucleopolyhedrovirus: scanning electron microscopy technique.

A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infect...

Aberration-Corrected Electron Microscopy of Nanoparticles

The very rich history of electron microscopy started with the discovery of the duality wave–particle. In the 1920s, the beginning of the twentieth century, it was shown that electrons could also behave as a wave. The optical microscopy based on Abbe’s theory already achieved the resolution limit ~ / ~ , λ 2 2 500Å. The electrons are located at high voltages and hence will have much shorter wave...

Direct detection pays off for electron cryo-microscopy

Improved electron detectors and image-processing techniques will allow the structures of macromolecules to be determined from tens of thousands of single-particle cryo-EM images, rather than the hundreds of thousands needed previously.

Electron Microscopy of Fowl Pox Virus

Scanning transmission electron microscopy methods for the analysis of nanoparticles.

Here we review the scanning transmission electron microscopy (STEM) characterization technique and STEM imaging methods. We describe applications of STEM for studying inorganic nanoparticles, and other uses of STEM in biological and health sciences and discuss how to interpret STEM results. The STEM imaging mode has certain benefits compared with the broad-beam illumination mode; the main advan...